Hepatic Granulomas in a Blood Python


Hepatic Granulomas in a Blood Python

History:  A 6 month old Blood Python was found dead with no apparent illness.

 

Gross Lesions: 

The snake was thin with prominent dorsal spinous processes of the vertebrae with mild autolysis. The stomach contained a partially digested mouse, and the colon was full of moderately firm feces. Near the vent was a 1 cm diameter yellow,doughy mass of urates.

 

Histopathology:

Liver: There are multifocal areas of necrosis with a central area filled with variable numbers of brightly eosinophilic degenerate and intact heterophils in a distinct mass with occasional cells having intracytoplasmic bacilli. Surrounding the heterophils are moderate numbers of macrophages and necrotic hepatocytes. 

 

Liver- Two heterophilic granulomas with hepatic necrosis and intralesional bacteria

Liver- Two heterophilic granulomas with hepatic necrosis and intralesional bacteria

 

 

Liver- Close up of a heterophilic granuloma. The bright red area in the center is a mass of degenerate heterophils.

Liver- Close up of a heterophilic granuloma. The bright red area in the center is a mass of degenerate heterophils. There is bacteria within the granuloma and around the edge. The surrounding hepatocytes are necrotic and there is infiltration by macrophages.

 

 

The submucosa is infiltrated by heterophils

Small Intestine: The submucosa is infiltrated by heterophils

 

 

 

 

Small Intestine: There is severe autolysis of the small intestine. Nevertheless there is a focal area of heterophilic and granulomatous inflammation with loss, blunting and flattening of villi. There are small numbers of heterogranulomas in the mucosa consisting of a central core of brightly eosinophilic heterophils surrounded by macrophages. There is

moderate amount of exudate and necrotic debris covering the mucosa with moderate numbers of bacilli colonies and multiple areas of mineralization. The submucosa has increased numbers of heterophils and macrophages.

 

 

Small Intestine- There is a clearly visible heterophilic granuloma in the necrotic debris in the lumen.  The mucosa is necrotic and sloughed.  All other sections of small and large intestine had autolyzed but discernible mucosal epithelium.

Small Intestine- There is a clearly visible heterophilic granuloma in the necrotic debris in the lumen. The mucosa is necrotic and sloughed. All other sections of small and large intestine had autolyzed but discernible mucosal epithelium.

 

 

Comment:

We did not culture the liver.  Common bacteria associted with these lesions are Salmonella sp, Aeromonas sp, and Pseudomonas sp among others.  Entamoeba invadens can also casue these lesions, but we did not see any of these organisms in the tissue sections.

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About Brian

Anatomic Pathologist, VetPath Services, Stone Ridge, NY- musculoskeletal, oral/dental, and sinonasal diseases
This entry was posted in Necropsy Cases and tagged , , . Bookmark the permalink.

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